ProST PEC1 ready-to-use (S3313S-500) is a specially developed medium for a serum- and xenofree in vitro culture of human progenitor endothelial cells (hPEC) containing all components necessary for optimal colony formation, clonogenic growth, and rapid proliferation. It is designed for use in an incubator at 37° C with a 5% CO2 atmosphere.
ProST PEC1 kit (S3312S-50) is provided with supplements (pre-screened and tested for progenitor cells) in separate sterile packing. This will enable the user to prepare a medium for special application. For example, VEGF, FGF-2, or other components may be omitted from the complete medium for specific experimental settings. Please note that such a formulation will not promote optimal cell growth. Therefore, this composition can not be used for routine long-term culture of PEC. Please make sure that sterility is not compromised when adding individual components to prepare complete medium. The medium should be carefully but thoroughly mixed after addition of all components to assure a homogeneous solution. Store basal or complete medium at 2 - 8° C and store supplements at -20° C. Expiry: 6 months.
Basal medium (w/o supplements) or complete/ready-to-use medium should not be frozen!
ProST PEC1 kit