Serum, produced from animal blood and fetal bovine serum, is the most widely used because it contains an especially high amount of growth factors due to its origin. Animal serum is a highly complex organic solution which contains a range of cellular components including vitamins, nutrients, nucleosides, amino acids, carbohydrates, hormones and more. Serum provides the necessary nutrition to a cell and is also an essential medium promoting most or all cellular functions. This is essential to a healthy cell culture such as mitosis, attachment and proliferation.
The function of serum in cell culture
- stimulate cell growth, proliferate and differentiate through hormonal factors
- adhesion factors facilitate/enhance cell attachment on culture dishes
- transport and binding proteins provide hormons, minerals and lipids
- bind toxic substances through serum proteins